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gRNA Sequence Heterology Tolerance Catalyzed by CRISPR/Cas in an In Vitro Homology-Directed Repair Reaction

CRISPR and associated Cas nucleases are genetic engineering tools revolutionizing innovative approaches to cancer and inherited diseases. CRISPR-directed gene editing relies heavily on proper DNA sequence alignment between the guide RNA (gRNA)/CRISPR complex and its genomic target. Accurate hybridiz...

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Detalhes bibliográficos
Publicado no:Mol Ther Nucleic Acids
Main Authors: Hewes, Amanda M., Sansbury, Brett M., Barth, Shaul, Tarcic, Gabi, Kmiec, Eric B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Gene & Cell Therapy 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7177190/
https://ncbi.nlm.nih.gov/pubmed/32330873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2020.03.012
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