Guide RNAs with embedded barcodes boost CRISPR-pooled screens

We report a new method using re-designed guide RNAs with internal barcodes (iBARs) embedded in their loop regions. Our iBAR approach outperforms the conventional method by producing screening results with much lower false-positive and false-negative rates especially with a high multiplicity of infec...

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Enregistré dans:
Détails bibliographiques
Publié dans:Genome Biol
Auteurs principaux: Zhu, Shiyou, Cao, Zhongzheng, Liu, Zhiheng, He, Yuan, Wang, Yinan, Yuan, Pengfei, Li, Wei, Tian, Feng, Bao, Ying, Wei, Wensheng
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2019
Sujets:
Method
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6345036/
https://ncbi.nlm.nih.gov/pubmed/30678704
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-019-1628-0
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