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Expression of CRISPR/Cas single guide RNAs using small tRNA promoters
The in vivo application of CRISPR/Cas-based DNA editing technology will require the development of efficient delivery methods that likely will be dependent on adeno-associated virus (AAV)-based viral vectors. However, AAV vectors have only a modest, ∼4.7-kb packaging capacity, which will necessitate...
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| Udgivet i: | RNA |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4536327/ https://ncbi.nlm.nih.gov/pubmed/26187160 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.051631.115 |
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