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An improved yeast surface display platform for the screening of nanobody immune libraries

Fusions to the C-terminal end of the Aga2p mating adhesion of Saccharomyces cerevisiae have been used in many studies for the selection of affinity reagents by yeast display followed by flow cytometric analysis. Here we present an improved yeast display system for the screening of Nanobody immune li...

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Publicado en:Sci Rep
Main Authors: Uchański, Tomasz, Zögg, Thomas, Yin, Jie, Yuan, Daopeng, Wohlkönig, Alexandre, Fischer, Baptiste, Rosenbaum, Daniel M., Kobilka, Brian K., Pardon, Els, Steyaert, Jan
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6344588/
https://ncbi.nlm.nih.gov/pubmed/30674983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-37212-3
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