In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian Cells

The CRISPR-Cas9 system is a powerful genome-editing tool that promises application for gene editing therapies. The Cas9 nuclease is directed to the DNA by a programmable single guide (sg)RNA, and introduces a site-specific double-stranded break (DSB). In mammalian cells, DSBs are either repaired by...

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Vydáno v:Bio Protoc
Hlavní autoři: Savić, Nataša, Ringnalda, Femke Cas, Berk, Christian, Bargsten, Katja, Hall, Jonathan, Jinek, Martin, Schwank, Gerald
Médium: Artigo
Jazyk:Inglês
Vydáno: Bio-Protocol 2019
Témata:
Methods Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6340478/
https://ncbi.nlm.nih.gov/pubmed/30675496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3136
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