Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

The CRISPR-Cas9 targeted nuclease technology allows the insertion of genetic modifications with single base-pair precision. The preference of mammalian cells to repair Cas9-induced DNA double-strand breaks via error-prone end-joining pathways rather than via homology-directed repair mechanisms, howe...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:eLife
Main Authors: Savic, Natasa, Ringnalda, Femke CAS, Lindsay, Helen, Berk, Christian, Bargsten, Katja, Li, Yizhou, Neri, Dario, Robinson, Mark D, Ciaudo, Constance, Hall, Jonathan, Jinek, Martin, Schwank, Gerald
Format: Artigo
Jezik:Inglês
Izdano: eLife Sciences Publications, Ltd 2018
Teme:
Chromosomes and Gene Expression
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6023611/
https://ncbi.nlm.nih.gov/pubmed/29809142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.33761
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