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Probing CRISPR-Cas12a Nuclease Activity Using Double-Stranded DNA-Templated Fluorescent Substrates

The CRISPR-Cas12a nuclease shreds short single-stranded DNA (ssDNA) substrates indiscriminately through transcleavage upon activation with a specific target DNA. This shredding activity offered the potential for development of ssDNA-templated probes with fluorescent dye (F) and quencher (Q) labels....

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Dades bibliogràfiques
Publicat a:Biochemistry
Autors principals: Smith, Christopher W., Nandu, Nidhi, Kachwala, Mahera J., Chen, Yu-Sheng, Uyar, Taha Bilal, Yigit, Mehmet V.
Format: Artigo
Idioma:Inglês
Publicat: 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7384386/
https://ncbi.nlm.nih.gov/pubmed/32233423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.0c00140
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