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Targeting individual cells by barcode in pooled sequence libraries

Transcriptional profiling of thousands of single cells in parallel by RNA-seq is now routine. However, due to reliance on pooled library preparation, targeting analysis to particular cells of interest is difficult. Here, we present a multiplexed PCR method for targeted sequencing of select cells fro...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Ranu, Navpreet, Villani, Alexandra-Chloé, Hacohen, Nir, Blainey, Paul C
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6326790/
https://ncbi.nlm.nih.gov/pubmed/30256981
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky856
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