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Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs

We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small...

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Hlavní autoři: Casbon, James A., Slatter, Andrew F., Musgrave-Brown, Esther, Osborne, Robert J., Lichtenstein, Conrad P., Brenner, Sydney
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664814/
https://ncbi.nlm.nih.gov/pubmed/23580546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt228
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