Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

BACKGROUND: Cultured human cells are pivotal models to study human gene functions, but introducing complete loss of function in diploid or aneuploid cells has been a challenge. The recently developed CRISPR/Cas9-mediated homology-independent knock-in approach permits targeted insertion of large DNA...

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Udgivet i:BMC Biol
Main Authors: Zhang, Chenzi, He, Xiangjun, Kwok, Yvonne K., Wang, Feng, Xue, Junyi, Zhao, Hui, Suen, Kin Wah, Wang, Chi Chiu, Ren, Jianwei, Chen, George G., Lai, Paul B. S., Li, Jiangchao, Xia, Yin, Chan, Andrew M., Chan, Wai-Yee, Feng, Bo
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2018
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6310992/
https://ncbi.nlm.nih.gov/pubmed/30593266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12915-018-0616-2
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