CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) is widely used for mediating the knock-in of foreign DNA into the genomes of various organisms. Here, we report a process of CRISPR/Cas-mediated knock-in via non-homologous end joining by the direct...

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Библиографические подробности
Опубликовано в: :PLoS One
Главные авторы: Kumagai, Hitoshi, Nakanishi, Takashi, Matsuura, Tomoaki, Kato, Yasuhiko, Watanabe, Hajime
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2017
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5646780/
https://ncbi.nlm.nih.gov/pubmed/29045453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0186112
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