ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous recombination...

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Publicat a:mBio
Autors principals: Murphy, Kenan C., Nelson, Samantha J., Nambi, Subhalaxmi, Papavinasasundaram, Kadamba, Baer, Christina E., Sassetti, Christopher M.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2018
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6299477/
https://ncbi.nlm.nih.gov/pubmed/30538179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.01467-18
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