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ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes
Two efficient recombination systems were combined to produce a versatile method for chromosomal engineering that obviates the need to prepare double-stranded DNA (dsDNA) recombination substrates. A synthetic “targeting oligonucleotide” is incorporated into the chromosome via homologous recombination...
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| Publicado no: | mBio |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6299477/ https://ncbi.nlm.nih.gov/pubmed/30538179 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.01467-18 |
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