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Essential role of polymerases for assay performance – Impact of polymerase replacement in a well-established assay

The quantitative real-time polymerase chain reaction (qPCR) is one of the most commonly molecular methods used today. It is central to numerous assays that have since been developed and described around its optimization. The Listeria monocytogenes prfA qPCR assay has been studied in great detail and...

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Detalhes bibliográficos
Publicado no:Biomol Detect Quantif
Main Authors: Witte, Anna Kristina, Sickha, Romana, Mester, Patrick, Fister, Susanne, Schoder, Dagmar, Rossmanith, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6287537/
https://ncbi.nlm.nih.gov/pubmed/30560063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2018.10.002
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