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A Systematic Investigation of Parameters Influencing Droplet Rain in the Listeria monocytogenes prfA Assay - Reduction of Ambiguous Results in ddPCR

The droplet digital polymerase chain reaction (ddPCR) determines DNA amounts based upon the pattern of positive and negative droplets, according to Poisson distribution, without the use of external standards. However, division into positive and negative droplets is often not clear because a part of...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Witte, Anna Kristina, Mester, Patrick, Fister, Susanne, Witte, Matthias, Schoder, Dagmar, Rossmanith, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5167268/
https://ncbi.nlm.nih.gov/pubmed/27992475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0168179
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