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Essential role of polymerases for assay performance – Impact of polymerase replacement in a well-established assay

The quantitative real-time polymerase chain reaction (qPCR) is one of the most commonly molecular methods used today. It is central to numerous assays that have since been developed and described around its optimization. The Listeria monocytogenes prfA qPCR assay has been studied in great detail and...

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Dades bibliogràfiques
Publicat a:Biomol Detect Quantif
Autors principals: Witte, Anna Kristina, Sickha, Romana, Mester, Patrick, Fister, Susanne, Schoder, Dagmar, Rossmanith, Peter
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6287537/
https://ncbi.nlm.nih.gov/pubmed/30560063
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2018.10.002
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