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A rapid fluorescent indicator displacement assay and principal component/cluster data analysis for determination of ligand–nucleic acid structural selectivity

We describe a rapid fluorescence indicator displacement assay (R-FID) to evaluate the affinity and the selectivity of compounds binding to different DNA structures. We validated the assay using a library of 30 well-known nucleic acid binders containing a variety chemical scaffolds. We used a combina...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: del Villar-Guerra, Rafael, Gray, Robert D, Trent, John O, Chaires, Jonathan B
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6283418/
https://ncbi.nlm.nih.gov/pubmed/29361140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky019
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