High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension

BACKGROUND: Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated by protein factors, and this regulation can impact on the levels of miRNAs and m...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Bouvette, Jonathan, Korkut, Dursun Nizam, Fouillen, Aurélien, Amellah, Soumiya, Nanci, Antonio, Durocher, Yves, Omichinski, James G., Legault, Pascale
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6282390/
https://ncbi.nlm.nih.gov/pubmed/30522464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-018-0485-3
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