High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

The art of producing recombinant proteins with complex post-translational modifications represents a major challenge for studies of structure and function. The rapid establishment and high recovery from transiently-transfected mammalian cell lines addresses this barrier and is an effective means of...

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Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Subedi, Ganesh P., Johnson, Roy W., Moniz, Heather A., Moremen, Kelley W., Barb, Adam
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2015
Teme:
Cellular Biology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4780855/
https://ncbi.nlm.nih.gov/pubmed/26779721
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53568
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