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A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors

An assay for telomerase activity based on asymmetric polymerase chain reaction (A-PCR) on magnetic beads (MBs) and subsequent application of cycling probe technology (CPT) is described. In this assay, the telomerase reaction products are immobilized on MBs, which are then washed to remove PCR inhibi...

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Publicat a:Molecules
Autors principals: Yaku, Hidenobu, Murashima, Takashi, Miyoshi, Daisuke, Sugimoto, Naoki
Format: Artigo
Idioma:Inglês
Publicat: MDPI 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6269933/
https://ncbi.nlm.nih.gov/pubmed/24071983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules181011751
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