A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors

An assay for telomerase activity based on asymmetric polymerase chain reaction (A-PCR) on magnetic beads (MBs) and subsequent application of cycling probe technology (CPT) is described. In this assay, the telomerase reaction products are immobilized on MBs, which are then washed to remove PCR inhibi...

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Hauptverfasser: Hidenobu Yaku, Takashi Murashima, Daisuke Miyoshi, Naoki Sugimoto
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI AG 2013-09-01
Schriftenreihe:Molecules
Schlagworte:
telomerase
PCR inhibitor
magnetic beads
asymmetric PCR
cycling probe technology
Online Zugang:http://www.mdpi.com/1420-3049/18/10/11751
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