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A Highly Sensitive Telomerase Activity Assay that Eliminates False-Negative Results Caused by PCR Inhibitors

An assay for telomerase activity based on asymmetric polymerase chain reaction (A-PCR) on magnetic beads (MBs) and subsequent application of cycling probe technology (CPT) is described. In this assay, the telomerase reaction products are immobilized on MBs, which are then washed to remove PCR inhibi...

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Bibliografiske detaljer
Udgivet i:Molecules
Main Authors: Yaku, Hidenobu, Murashima, Takashi, Miyoshi, Daisuke, Sugimoto, Naoki
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6269933/
https://ncbi.nlm.nih.gov/pubmed/24071983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules181011751
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