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A novel cloning strategy for one-step assembly of multiplex CRISPR vectors
One key advantage of the CRISPR/Cas9 system in comparison with other gene editing approaches lies in its potential for multiplexing. Here, we describe an elaborate procedure that allows the assembly of multiple gRNA expression cassettes into a vector of choice within a single step, termed ASAP(Adapt...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6269432/ https://ncbi.nlm.nih.gov/pubmed/30504793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-35727-3 |
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