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Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only for single gene targeting, but also for multiple targeted mutagenesis. To make the most of the multiplexity of CRISPR/Cas9, we established a system for constructing all-in-one expression vectors con...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Publishing Group
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4066266/ https://ncbi.nlm.nih.gov/pubmed/24954249 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep05400 |
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