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Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression

CRISPR/Cas9 technology enables efficient, rapid and cost-effective targeted genomic modification in a wide variety of cellular contexts including cultured cells. Some applications such as generation of double knock-outs, large deletions and paired-nickase cleavage require simultaneous expression of...

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Podrobná bibliografie
Vydáno v:PLoS One
Hlavní autoři: Adikusuma, Fatwa, Pfitzner, Chandran, Thomas, Paul Quinton
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5718404/
https://ncbi.nlm.nih.gov/pubmed/29211736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0187236
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