One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time. A significant advantage of CRISPR over previous methods is its tremendous adaptability due to its bipartite nature. Cas9 or its engineered v...

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Detalles Bibliográficos
Publicado en:PLoS One
Main Authors: Breunig, Christopher T., Durovic, Tamara, Neuner, Andrea M., Baumann, Valentin, Wiesbeck, Maximilian F., Köferle, Anna, Götz, Magdalena, Ninkovic, Jovica, Stricker, Stefan H.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2018
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5922533/
https://ncbi.nlm.nih.gov/pubmed/29702666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196015
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