One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)

Novel applications based on the bacterial CRISPR system make genetic, genomic, transcriptional and epigenomic engineering widely accessible for the first time. A significant advantage of CRISPR over previous methods is its tremendous adaptability due to its bipartite nature. Cas9 or its engineered v...

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Bibliographic Details
Published in:PLoS One
Main Authors: Breunig, Christopher T., Durovic, Tamara, Neuner, Andrea M., Baumann, Valentin, Wiesbeck, Maximilian F., Köferle, Anna, Götz, Magdalena, Ninkovic, Jovica, Stricker, Stefan H.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2018
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5922533/
https://ncbi.nlm.nih.gov/pubmed/29702666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196015
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