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A sensitive high throughput screening method for identifying small molecule stimulators of the core particle of the proteasome

Fluorescence resonance energy transfer (FRET) technology is a useful tool to monitor protein interactions as well as protease activity. We have recently reported a biochemical assay utilizing a FRET reporter peptide to monitor the activity of the 20S catalytic particle (20S CP) of the proteasome. Th...

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Bibliografische gegevens
Gepubliceerd in:Curr Protoc Chem Biol
Hoofdauteurs: Coleman, Rachel A., Trader, Darci J.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6263736/
https://ncbi.nlm.nih.gov/pubmed/30285317
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.52
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