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A sensitive high throughput screening method for identifying small molecule stimulators of the core particle of the proteasome
Fluorescence resonance energy transfer (FRET) technology is a useful tool to monitor protein interactions as well as protease activity. We have recently reported a biochemical assay utilizing a FRET reporter peptide to monitor the activity of the 20S catalytic particle (20S CP) of the proteasome. Th...
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| Опубликовано в: : | Curr Protoc Chem Biol |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6263736/ https://ncbi.nlm.nih.gov/pubmed/30285317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpch.52 |
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