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Generation of Stable Expression Mammalian Cell Lines Using Lentivirus

Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell lines using 3(rd) generation lentivirus is very rob...

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Bibliografski detalji
Izdano u:Bio Protoc
Glavni autori: Tandon, Neha, Thakkar, Kaushik N., LaGory, Edward L, Liu, Yu, Giaccia, Amato J
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2018
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6261361/
https://ncbi.nlm.nih.gov/pubmed/30505888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3073
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