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Generation of Stable Expression Mammalian Cell Lines Using Lentivirus
Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell lines using 3(rd) generation lentivirus is very rob...
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| 出版年: | Bio Protoc |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Bio-Protocol
2018
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6261361/ https://ncbi.nlm.nih.gov/pubmed/30505888 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3073 |
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