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Generating Mammalian Stable Cell Lines by Electroporation

Expression of functional, recombinant mammalian proteins often requires expression in mammalian cells (see Single Cell Cloning of a Stable Mammalian Cell Line). If the expressed protein needs to be made frequently, it can be best to generate a stable cell line instead of performing repeated transien...

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Detalhes bibliográficos
Main Authors: Longo, Patti A., Kavran, Jennifer M., Kim, Min-Sung, Leahy, Daniel J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4088271/
https://ncbi.nlm.nih.gov/pubmed/24011048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-418687-3.00017-3
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