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Generating Mammalian Stable Cell Lines by Electroporation
Expression of functional, recombinant mammalian proteins often requires expression in mammalian cells (see Single Cell Cloning of a Stable Mammalian Cell Line). If the expressed protein needs to be made frequently, it can be best to generate a stable cell line instead of performing repeated transien...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4088271/ https://ncbi.nlm.nih.gov/pubmed/24011048 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-418687-3.00017-3 |
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