Single step production of Cas9 mRNA for zygote injection

Production of Cas9 mRNA in vitro typically requires the addition of a 5ʹ cap and 3ʹ polyadenylation. A plasmid was constructed that harbored the T7 promoter followed by the EMCV IRES and a Cas9 coding region. We hypothesized that the use of the metastasis associated lung adenocarcinoma transcript 1...

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Detalhes bibliográficos
Publicado no:Biotechniques
Main Authors: Redel, Bethany K, Beaton, Benjamin P, Spate, Lee D, Benne, Joshua A, Murphy, Stephanie L, O’ Gorman, Chad W, Spate, Anna M, Prather, Randall S, Wells, Kevin D
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6240161/
https://ncbi.nlm.nih.gov/pubmed/29570443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2017-0116
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