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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Characterization of the clustered, regularly interspaced, short, palindromic repeat (CRISPR) system of Streptococcus pyogenes has enabled the development of a customizable platform to rapidly generate gene modifications in a wide variety of organisms, including zebrafish. CRISPR-based genome editing...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Sorlien, Erin L., Witucki, Mary A., Ogas, Joseph
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6231919/
https://ncbi.nlm.nih.gov/pubmed/30222157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56969
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