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Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans
CRISPR-based genome editing using ribonucleoprotein complexes and synthetic single-stranded oligodeoxynucleotide (ssODN) donors can be highly effective. However, reproducibility can vary, and precise, targeted integration of longer constructs—such as green fluorescent protein tags remains challengin...
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| Udgivet i: | Genetics |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Genetics Society of America
2018
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6218216/ https://ncbi.nlm.nih.gov/pubmed/30213854 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.118.301532 |
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