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RNA Interference in Caenorhabditis Elegans

RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.

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Detalhes bibliográficos
Publicado no:Curr Protoc Mol Biol
Main Authors: Conte, Darryl, MacNeil, Lesley T., Walhout, Albertha J.M., Mello, Craig C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5396541/
https://ncbi.nlm.nih.gov/pubmed/25559107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb2603s109
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