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RNA Interference in Caenorhabditis Elegans

RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.

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Bibliografische gegevens
Gepubliceerd in:Curr Protoc Mol Biol
Hoofdauteurs: Conte, Darryl, MacNeil, Lesley T., Walhout, Albertha J.M., Mello, Craig C.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2015
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5396541/
https://ncbi.nlm.nih.gov/pubmed/25559107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb2603s109
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