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Measurement of Kinetics and Active Site Distances in Metalloenzyme Using Paramagnetic NMR with (13)C Hyperpolarization

Paramagnetic relaxation enhancement (PRE) conjoint with hyperpolarized NMR reveals structural information on the enzyme-product complex in an on-going metalloenzyme catalyzed reaction. Substrates of pseudouridine monophosphate glycosidase are hyperpolarized using the dynamic nuclear polarization (DN...

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Detalhes bibliográficos
Publicado no:J Phys Chem Lett
Main Authors: Liu, Mengxiao, Zhang, Guannan, Mahanta, Nilkamal, Lee, Youngbok, Hilty, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6200339/
https://ncbi.nlm.nih.gov/pubmed/29624056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jpclett.8b00350
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