One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination...

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Bibliographic Details
Published in:J Exp Med
Main Authors: Jacobsen, Johanne T., Mesin, Luka, Markoulaki, Styliani, Schiepers, Ariën, Cavazzoni, Cecília B., Bousbaine, Djenet, Jaenisch, Rudolf, Victora, Gabriel D.
Format: Artigo
Language:Inglês
Published: Rockefeller University Press 2018
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Research Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6170169/
https://ncbi.nlm.nih.gov/pubmed/30181412
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1084/jem.20172064
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