Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

BACKGROUND: Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between efficiency, specificity and sensitivity in the ampl...

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發表在:BMC Bioinformatics
Main Authors: Sambo, Francesco, Finotello, Francesca, Lavezzo, Enrico, Baruzzo, Giacomo, Masi, Giulia, Peta, Elektra, Falda, Marco, Toppo, Stefano, Barzon, Luisa, Di Camillo, Barbara
格式: Artigo
語言:Inglês
出版: BioMed Central 2018
主題:
Methodology Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6162885/
https://ncbi.nlm.nih.gov/pubmed/30268091
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-018-2360-6
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