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Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene
BACKGROUND: Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between efficiency, specificity and sensitivity in the ampl...
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| Publié dans: | BMC Bioinformatics |
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| Auteurs principaux: | , , , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6162885/ https://ncbi.nlm.nih.gov/pubmed/30268091 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-018-2360-6 |
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