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Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

BACKGROUND: Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between efficiency, specificity and sensitivity in the ampl...

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Détails bibliographiques
Publié dans:BMC Bioinformatics
Auteurs principaux: Sambo, Francesco, Finotello, Francesca, Lavezzo, Enrico, Baruzzo, Giacomo, Masi, Giulia, Peta, Elektra, Falda, Marco, Toppo, Stefano, Barzon, Luisa, Di Camillo, Barbara
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2018
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6162885/
https://ncbi.nlm.nih.gov/pubmed/30268091
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-018-2360-6
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