Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

BACKGROUND: Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this approach strongly depends on the choice of primer pairs and, in particular, on the balance between efficiency, specificity and sensitivity in the ampl...

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Bibliografiske detaljer
Udgivet i:BMC Bioinformatics
Main Authors: Sambo, Francesco, Finotello, Francesca, Lavezzo, Enrico, Baruzzo, Giacomo, Masi, Giulia, Peta, Elektra, Falda, Marco, Toppo, Stefano, Barzon, Luisa, Di Camillo, Barbara
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2018
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6162885/
https://ncbi.nlm.nih.gov/pubmed/30268091
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-018-2360-6
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