Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene

Genetic code expansion enables the incorporation of non-canonical amino acids (ncAAs) into expressed proteins. ncAAs are usually encoded by a stop codon that is decoded by an exogenous orthogonal aminoacyl tRNA synthetase and its cognate suppressor tRNA, such as the pyrrolysine [Formula: see text] p...

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Vydáno v:Sci Rep
Hlavní autoři: Aloush, Noa, Schvartz, Tomer, König, Andres I., Cohen, Sarit, Brozgol, Eugene, Tam, Benjamin, Nachmias, Dikla, Ben-David, Oshrit, Garini, Yuval, Elia, Natalie, Arbely, Eyal
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2018
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6162220/
https://ncbi.nlm.nih.gov/pubmed/30267004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-32824-1
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