A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag

BACKGROUND: In the high-resolution microscopy era, genetic code expansion (GCE)-based bioorthogonal labeling offers an elegant way for direct labeling of proteins in live cells with fluorescent dyes. This labeling approach is currently not broadly used in live-cell applications, partly because it ne...

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Detalhes bibliográficos
Publicado no:BMC Biol
Main Authors: Segal, Inbar, Nachmias, Dikla, Konig, Andres, Alon, Ariel, Arbely, Eyal, Elia, Natalie
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6961407/
https://ncbi.nlm.nih.gov/pubmed/31937312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12915-019-0708-7
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