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Orthogonality of Pyrrolysine tRNA in the Xenopus oocyte

Chemical aminoacylation of orthogonal tRNA allows for the genetic encoding of a wide range of synthetic amino acids without the need to evolve specific aminoacyl-tRNA synthetases. This method, when paired with protein expression in the Xenopus laevis oocyte expression system, can extract atomic scal...

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發表在:Sci Rep
Main Authors: Infield, Daniel T., Lueck, John D., Galpin, Jason D., Galles, Grace D., Ahern, Christopher A.
格式: Artigo
語言:Inglês
出版: Nature Publishing Group UK 2018
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5980078/
https://ncbi.nlm.nih.gov/pubmed/29581437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-23201-z
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