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In silico sgRNA tool design for CRISPR control of quorum sensing in Acinetobacter species
CRISPR genome editing utilizes Cas9 nuclease and single guide RNA (sgRNA), which directs the nuclease to a specific site in the genome and makes a double-stranded break (DSB). Design of sgRNA for CRISPR-Cas targeting, and to promote CRISPR adaptation, uses a regulatory mechanism that ensures maximum...
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| 發表在: | Genes Dis |
|---|---|
| Main Authors: | , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Chongqing Medical University
2018
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6146548/ https://ncbi.nlm.nih.gov/pubmed/30258941 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gendis.2018.03.004 |
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