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In silico sgRNA tool design for CRISPR control of quorum sensing in Acinetobacter species

CRISPR genome editing utilizes Cas9 nuclease and single guide RNA (sgRNA), which directs the nuclease to a specific site in the genome and makes a double-stranded break (DSB). Design of sgRNA for CRISPR-Cas targeting, and to promote CRISPR adaptation, uses a regulatory mechanism that ensures maximum...

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Detalhes bibliográficos
Publicado no:Genes Dis
Main Authors: Karlapudi, Abraham Peele, T.C, Venkateswarulu, Tammineedi, Jahnavi, Srirama, Krupanidhi, Kanumuri, Lohit, Prabhakar Kodali, Vidya
Formato: Artigo
Idioma:Inglês
Publicado em: Chongqing Medical University 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6146548/
https://ncbi.nlm.nih.gov/pubmed/30258941
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gendis.2018.03.004
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