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Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site

We have obtained new crystal structures of Mycobacterium tuberculosis topoisomerase I, including structures with ssDNA substrate bound to the active site, with and without Mg(2+) ion present. Significant enzyme conformational changes upon DNA binding place the catalytic tyrosine in a pre-transition...

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Bibliografski detalji
Izdano u:Nucleic Acids Res
Glavni autori: Cao, Nan, Tan, Kemin, Annamalai, Thirunavukkarasu, Joachimiak, Andrzej, Tse-Dinh, Yuk-Ching
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2018
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Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101483/
https://ncbi.nlm.nih.gov/pubmed/29905859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky492
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