Rare cell detection by single cell RNA sequencing as guided by single molecule RNA FISH

Although single cell RNA sequencing can reliably detect large-scale transcriptional programs, it is unclear whether it accurately captures the behavior of individual genes, especially those that express only in rare cells. Here, we use single molecule RNA FISH as a gold standard to assess tradeoffs...

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Vydáno v:Cell Syst
Hlavní autoři: Torre, Eduardo, Dueck, Hannah, Shaffer, Sydney, Gospocic, Janko, Gupte, Rohit, Bonasio, Roberto, Kim, Junhyong, Murray, John, Raj, Arjun
Médium: Artigo
Jazyk:Inglês
Vydáno: 2018
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6078200/
https://ncbi.nlm.nih.gov/pubmed/29454938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cels.2018.01.014
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