A protocol for introduction of multiple genetic modifications in Saccharomyces cerevisiae using CRISPR/Cas9

Here, two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method us...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:FEMS Yeast Res
Main Authors: Mans, Robert, Wijsman, Melanie, Daran-Lapujade, Pascale, Daran, Jean-Marc
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2018
Teme:
Protocol
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6074844/
https://ncbi.nlm.nih.gov/pubmed/29860374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsyr/foy063
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