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A protocol for introduction of multiple genetic modifications in Saccharomyces cerevisiae using CRISPR/Cas9
Here, two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method us...
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| Publicado no: | FEMS Yeast Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6074844/ https://ncbi.nlm.nih.gov/pubmed/29860374 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsyr/foy063 |
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